NanoDrop™ One Microvolume UV-Vis Spectrophotometer

Prevent costly delays with an enhanced understanding of sample quality using the Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometers. Innovative Thermo Scientific™ Acclaro™ Sample Intelligence technology is built into every instrument for improved measurement accuracy and contaminant identification.


  • Contains both pedestal and cuvette positions to expand experimental flexibility and increase the dynamic range.
  • Measure dilute samples, perform kinetics experiments and take optical density measurements of bacterial cultures.
  • Cuvette position includes temperature control and stirring.
Brand: Thermo Scientific™

Product Overview


Quantify and qualify DNA, RNA, and protein samples in seconds with only 1-2 μL, and obtain full-spectral data before you decide to use samples in downstream applications. Additional cuvette position also available.
Move one step closer to your research goals with an ergonomic, standalone spectrophotometer with high-resolution, touch screen interface. Powerful auto-range pathlength technology facilitates accurate measurements for concentrated samples with no need for dilutions. This is your innovative alternative to the NanoDrop 2000/2000c Spectrophotometers.


Acclaro Sample Intelligence Technology
Acclaro technology enhances user understanding of sample quality while delivering accurate quantitative measurements. Early identification of contaminants can prevent failure of downstream applications and save days of troubleshooting.
  • Verify sample quality in seconds – identify sample contaminants and obtain corrected concentration results.
  • Access information through alerts – receive instant feedback about sample quality with on-demand technical support and guided troubleshooting.
  • Rely on accurate results – ensure measurement integrity with embedded sensor and digital image analysis.
  • Identify nucleic acid contaminants such as RNA contamination in dsDNA samples and chemical contaminants such as phenol in RNA samples.
NanoDrop One/Nanodrop OneC PC Software with 21 CFR Part 11 Compliance
  • Optional Thermo Scientific Security Suite for NanoDrop One/OneC allows the instrument to be used in a 21 CFR Part 11 compliant lab. Features include:
  • User account access
    Audit trail
    Electronic signatures
    Automated data back-up, and more
NanoDrop One/NanoDrop OneC Enhancements
  • Ergonomic, standalone design – integrated Android™ tablet eliminates the need for a separate computer and provides seamless data transfer via Wireless*, Ethernet or USB.
  • Auto-Measure and Auto-Blank functions – multi-sample processing can be streamlined with instant measurements that occur when the pedestal arm is down. These functions can be turned ON or OFF with a single tap on the screen.
  • Wider dynamic range – no dilutions required for highly concentrated samples (dsDNA at 27,500 ng/μL) with auto-range pathlength technology
  • Integrated Learning Center – an archive of technical support documents and educational animations at your fingertips.
Advanced Connectivity for Data Storage, Printing and Sharing
  • Multiple choices for data export: transfer results with a USB, Wi-Fi or Ethernet connection to your PC or save data directly to the Thermo Fisher Cloud via Wi-Fi or Ethernet network.
  • View, organize and share data on a PC: Once exported, you can view the full-data formats (e.g., contaminant analysis spectral graphs) and organize experiments using the NanoDrop One/OneC PC Control software.
  • Easy data-to-printer options: Print your results directly from the NanoDrop One instrument via Wi-Fi, Ethernet or USB printer connection; print exported data from your PC using the Nanodrop One/OneC PC Control software.
  • Online collaborations: Share data between colleagues who do not have an instrument and view NanoDrop One data across multiple Thermo Fisher Cloud-connected accounts.
NanoDrop One© Highlights
  • Contains both pedestal and cuvette positions to expand experimental flexibility and increase the dynamic range.
  • Measure dilute samples, perform kinetics experiments and take optical density measurements of bacterial cultures.
  • Cuvette position includes temperature control and stirring.
NanoDrop One/NanoDrop OneC Capabilities
  • Wide spectral range (190-850 nm) for measuring a variety of samples types:
  • Peptides (205 nm)
    DNA and RNA (260 nm)
    Purified protein (280 nm)
    Toxicology assays and industrial dyes (490 nm)
    Gold nanoparticles (520 nm)
    Colorimetric protein assays (BCA 562 nm, Bradford 595 nm, Modified Lowry 650 nm, Pierce 660 660 nm)
    Optical Density measurements (600 nm)
  • Combining the patented** sample-retention system with cuvette capability accommodates low and high concentrations (2.0 – 27,500 ng/μL dsDNA and 0.06 – 820 mg/mL BSA)
  • Pedestal measurements that require only 1 – 2 μL of your sample and do not require sample dilution, even for highly concentrated samples
  • Calculates sample purity ratios (A260/A280 nm and A260/A230 nm)
  • Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry
  • User-friendly software includes custom methods and data export capabilities


Certifications/Compliance All NanoDrop instruments are approved to CE and UL/CSA.
Compatibility DYMO LabelWriter 450 printer, Bluetooth keyboard, mouse and barcode reader
Concentration dsDNA: Pedestal: 27,500 ng/μL
BSA (IgG): Pedestal: 820 (400) mg/mL
Connections Three USB-A ports, Ethernet, Bluetooth™ and Wi-Fi
(Only available on instruments with Wi-Fi/Bluetooth support)
Description NanoDrop OneC Microvolume UV-Vis Spectrophotometer with Wi-Fi
Detection Range dsDNA: Pedestal: 2.0 ng/μL, Cuvette: 0.2 ng/μL,
BSA (IgG): Pedestal: 0.06 (0.03) mg/mL, Cuvette: 0.006 (0.003) mg/mL
Detector Type 2048-element CMOS Linear Image Sensor
Display 7 in. High Definition Color Display, 1280 x 800 pixels
Dimensions (L x W x H) 20 x 25.4 x 32.3 cm (8 x 10 x 12.7 in.)
Languages English, French, German, Spanish, Polish, Chinese, Japanese, Korean
Light Source Xenon Flash
Spectral Bandwidth ≤1.8 nm (FWHM at Hg 254 nm)
Voltage 12 VDC
Wavelength Range 190 to 850 nm
For Use With (Application) Nucleic Acid A260, A260/A280, A260/A230 and Labeled Nucleic Acids;, Protein A280 and A205, Protein Pierce 660, Protein Bradford, Protein BCA, Protein Lowry, Labeled Proteins,
OD600, Kinetics, UV-Vis, and Custom Methods
Keypad Built-in touchscreen, multipoint capacitive touch
Measurement Time 8 seconds
Pathlength 0.030 to 1.0 mm (auto-ranging)
Photometric Accuracy 3% at 0.97 A, 302 nm, (Absorbance expressed at Abs/mm at 25°C)
Photometric Range 10 mm equivalent: Pedestal: 0–550 A; Cuvette: 0–1.5 A
Power Consumption Operating: 12 to 18 W; Standby: 5 W
Processor Android™
Repeatability Typical: 0.002 A (1.0 mm path) or 1%CV, whichever is greater
(SD of 10 individual measurements at 0.97 A)
Sample Temperature Temperature Control (cuvette only) 37 °C
Sample Volume (Metric) Minimum 1μL
System Requirements Windows™ 8.1 and 10, 64 bit
Wavelength Accuracy ±1 nm
Weight (English) 7.9 lb.
Weight (Metric) 3.6 kg

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